| Unlockable Duelist |
How to Unlock Them |
| Adrian Gecko Disk |
Beat the Exodius the Ultimate Forbidden One seal. |
| Alchemy Duel Disk |
Beat Jaden Yuki 10 times. |
| Alexis Rhodes - CPU Duel |
Complete all five of Guardian Savan's tests in World 2. |
| Amazoness Card Set |
Beat Amazoness Paladin using all Structure Decks. |
| Amazoness Duel Disk |
Beat Amazoness Paladin using all Structure Decks. |
| Aquarian Alessa - CPU Duel |
Beat Maiden of the Aqua underwater in World 2 five times. |
| Atticus Rhodes in "CPU Duel" |
Complete the Neo-Daedalus challenge in World 2. |
| Bastion Misawa |
Beat Amazoness Paladin with all 13 Structure Decks. |
| Chrysalis Dolphin - CPU Duel |
Beat Abyss Soldier underwater in World 2 five times. |
| Curse Of Darkness in World Championship Mode |
Talk to Gigobyte then Beat Curse of Darkness in World 1. |
| Curse of Vampire |
Beat Curse of Vampire in World of Grace. |
| D.D. Warrior Lady in "CPU Duel" |
Beat Warrior Lady of the Wasteland, in World 2, five times. |
| Dark Scorpion-Meanae the Thorn - CPU Duel |
Beat Don Zaloog in the pyramid in World 2 five times. |
| E- Hero Lady Heat |
Beat E-Hero Knospe five times. |
| Gear Golem the Moving Fortress - CPU Duel |
Beat Stronghold the Moving Fortress in World 3 five times. |
| Gorgeous Duel Disk |
Beat Jesse Anderson 10 times. |
| Gravekeeper's Chief - CPU Duel |
Beat Gravekeeper's Commandant in the pyramid in World 2 five times. |
| Hino Kagu-Tsuchi - CPU Duel |
Beat Dark Dust Spirit in the pyramid in World 2 five times. |
| Il Blud in "CPU Duel" |
Beat Blazewing Butterfly five times. |
| Kaibaman Duel Disk |
Beat Seto Kaiba 10 times. |
| Kaiser Sea Horse - CPU Duel |
Beat Sea Serpent Warrior of Darkness underwater in World 2 five times. |
| King of the Skull Servants |
Beat Skull Servant five times. |
| Luster Dragon #2 |
Beat Luster Dragon in the Civilization World five times. |
| Marcel Bonaparte in "CPU Duel" |
Complete the Exxod challenge in World 2. |
| Marie the Fallen One |
Beat Absorbing Kid from the Sky in World 5, five times. |
| Maximillion Pegasus in World Championship Mode |
Beat all 5 Guardian Sphinx challenges in World 2. |
| Maximillon Pegasus |
Clear all 5 of Guardian Sphinx's Duel Puzzles. |
| Ojama Yellow and Blowback Dragon |
Beat Blowback Dragon in World of Civilization. |
| Otohime |
Beat Otohime in Underwater (World of Sunlight). |
| Reaper on the Nightmare in "CPU Duel" |
Beat Nightmare Penguin, in World 1, five times. |
| Sabersaurus in "CPU Duel" |
Beat Kabazauls, in World 2, five times. |
| Sand Moth |
Beat Sand Moth in World of Sunlight. |
| Slipheed in "CPU Duel" |
Beat Storm Shooter, in world 1, five times. |
| Spirit of the Pharaoh |
Beat Spirit of the Pharaon in the Pyramid (World of Sunlight). |
| Syrus Truesdale in World Championship Mode |
Beat all five Guardian - Embust challenges in world 1. |
| Tag Duels |
Beat the first 3 worlds. |
| The Unhappy Maiden - CPU Duel |
Give "The Unhappy Maiden" five Life Point restoring cards in World 3. |
| Tournament Mode |
Unlock all duelists in World Championship mode to gain access of the Tournament Mode. |
| Unlock Sand Moth - CPU Duel mode |
Help out Pyramid Turtle on the World 2 map, then Beat Sand Moth in a duel. |
| Water Dragon |
Beat Kairyu-shin five times. |
| Woodborg Inpachi |
Beat all three Inpachis in World of Civilization. |
| Yellow Duel Disk |
Beat all World 1 opponents 2 times. |
| Yellow Uniform |
Beat the first four duelists in Free Tag Duel. |