| Unlockable Duelist | How to Unlock Them |
| Adrian Gecko Disk | Beat the Exodius the Ultimate Forbidden One seal. |
| Alchemy Duel Disk | Beat Jaden Yuki 10 times. |
| Alexis Rhodes - CPU Duel | Complete all five of Guardian Savan's tests in World 2. |
| Amazoness Card Set | Beat Amazoness Paladin using all Structure Decks. |
| Amazoness Duel Disk | Beat Amazoness Paladin using all Structure Decks. |
| Aquarian Alessa - CPU Duel | Beat Maiden of the Aqua underwater in World 2 five times. |
| Atticus Rhodes in "CPU Duel" | Complete the Neo-Daedalus challenge in World 2. |
| Bastion Misawa | Beat Amazoness Paladin with all 13 Structure Decks. |
| Chrysalis Dolphin - CPU Duel | Beat Abyss Soldier underwater in World 2 five times. |
| Curse Of Darkness in World Championship Mode | Talk to Gigobyte then Beat Curse of Darkness in World 1. |
| Curse of Vampire | Beat Curse of Vampire in World of Grace. |
| D.D. Warrior Lady in "CPU Duel" | Beat Warrior Lady of the Wasteland, in World 2, five times. |
| Dark Scorpion-Meanae the Thorn - CPU Duel | Beat Don Zaloog in the pyramid in World 2 five times. |
| E- Hero Lady Heat | Beat E-Hero Knospe five times. |
| Gear Golem the Moving Fortress - CPU Duel | Beat Stronghold the Moving Fortress in World 3 five times. |
| Gorgeous Duel Disk | Beat Jesse Anderson 10 times. |
| Gravekeeper's Chief - CPU Duel | Beat Gravekeeper's Commandant in the pyramid in World 2 five times. |
| Hino Kagu-Tsuchi - CPU Duel | Beat Dark Dust Spirit in the pyramid in World 2 five times. |
| Il Blud in "CPU Duel" | Beat Blazewing Butterfly five times. |
| Kaibaman Duel Disk | Beat Seto Kaiba 10 times. |
| Kaiser Sea Horse - CPU Duel | Beat Sea Serpent Warrior of Darkness underwater in World 2 five times. |
| King of the Skull Servants | Beat Skull Servant five times. |
| Luster Dragon #2 | Beat Luster Dragon in the Civilization World five times. |
| Marcel Bonaparte in "CPU Duel" | Complete the Exxod challenge in World 2. |
| Marie the Fallen One | Beat Absorbing Kid from the Sky in World 5, five times. |
| Maximillion Pegasus in World Championship Mode | Beat all 5 Guardian Sphinx challenges in World 2. |
| Maximillon Pegasus | Clear all 5 of Guardian Sphinx's Duel Puzzles. |
| Ojama Yellow and Blowback Dragon | Beat Blowback Dragon in World of Civilization. |
| Otohime | Beat Otohime in Underwater (World of Sunlight). |
| Reaper on the Nightmare in "CPU Duel" | Beat Nightmare Penguin, in World 1, five times. |
| Sabersaurus in "CPU Duel" | Beat Kabazauls, in World 2, five times. |
| Sand Moth | Beat Sand Moth in World of Sunlight. |
| Slipheed in "CPU Duel" | Beat Storm Shooter, in world 1, five times. |
| Spirit of the Pharaoh | Beat Spirit of the Pharaon in the Pyramid (World of Sunlight). |
| Syrus Truesdale in World Championship Mode | Beat all five Guardian - Embust challenges in world 1. |
| Tag Duels | Beat the first 3 worlds. |
| The Unhappy Maiden - CPU Duel | Give "The Unhappy Maiden" five Life Point restoring cards in World 3. |
| Tournament Mode | Unlock all duelists in World Championship mode to gain access of the Tournament Mode. |
| Unlock Sand Moth - CPU Duel mode | Help out Pyramid Turtle on the World 2 map, then Beat Sand Moth in a duel. |
| Water Dragon | Beat Kairyu-shin five times. |
| Woodborg Inpachi | Beat all three Inpachis in World of Civilization. |
| Yellow Duel Disk | Beat all World 1 opponents 2 times. |
| Yellow Uniform | Beat the first four duelists in Free Tag Duel. |